![proteus for mplab x ide 4.05 proteus for mplab x ide 4.05](https://microchipdeveloper.com/local--files/install-i:proteus-win/Run.png)
Furthermore, five rape samples with unknown thiabendazole pesticides concentration were used to verify the accuracy and reliability of this method. The prediction effect of thiabendazole pesticides in rape was the best (Rp2 = 0.94, RMSEP = 3.17 mg/L) after the original spectra preprocessed with 1st-Derivative, and the linear relevance between thiabendazole pesticides concentration and Raman peak intensity at 782 cm−1 was the highest (R2 = 0.91). Moreover, 782, 10 cm−1 could be determined as thiabendazole pesticides Raman characteristic peaks in rape. As a result, the SERS enhancing effect based on silver Nano-substrate was better than that of gold Nano-substrate, where the detection limit of thiabendazole pesticides in rape could reach 0.1 mg/L.
![proteus for mplab x ide 4.05 proteus for mplab x ide 4.05](https://microcontrollerslab.com/wp-content/uploads/2017/08/MPLAB-XC8-Compiler-select-xc8-compiler.jpg)
The original SERS were pretreated and the partial least squares (PLS) was applied to establish the prediction model between SERS and thiabendazole pesticides in rape. In this paper, a rapid determination method of thiabendazole pesticides in rape was conducted combining SERS with chemometric methods.
![proteus for mplab x ide 4.05 proteus for mplab x ide 4.05](https://img.informer.com/pd/mplab-x-ide-v4-main-window-outlook.png)
Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive fingerprint with the advantages of simple operation, convenient portability and high detection efficiency. Accurate monitoring of thiabendazole pesticides in plants will prevent potential adverse effects to the Environment and human health. Thiabendazole is widely used in sclerotium blight, downy mildew and black rot prevention and treatment in rape. Lin, Lei Nie, Pengcheng Qu, Fangfang Chu, Bingquan Xiao, Shupei Rapid Determination of Thiabendazole Pesticides in Rape by Surface Enhanced Raman Spectroscopy